
( Brand: Chroma ), ( Manufacturer Part Number: 61008 ), ( Part Type: Filter ), ( Filter Size: 18mm )
The Chroma 61008 CFP/YFP/dsRed 630/60 Filter is a high-performance optical filter designed for fluorescence microscopy, offering exceptional versatility and precision for researchers working with various fluorescent proteins.
This filter set is specifically tailored for simultaneous excitation of the Cy5 (CFP), Citrine (YFP), and DsRed fluorescent proteins. The 630/60 nm bandpass emission filter ensures efficient suppression of excitation light and fluorescence from irrelevant wavelengths, while allowing for the collection of signals from the targeted fluorophores.
The 436/20 nm bandpass excitation filter is optimized for excitation of the CFP fluorescent protein, minimizing cross-talk between excitation and emission spectra. Similarly, the 500/25 nm bandpass excitation filter is designed for efficient excitation of the YFP fluorescent protein, while the 562/36 nm bandpass excitation filter ensures strong excitation of the DsRed fluorescent protein.
Built with Chroma's renowned optical quality, the 61008 filter set provides exceptional contrast and resolution, ensuring the highest possible image quality. These filters are made using Chroma's proprietary multi-layer coating technology, ensuring superior flatness, parallelism, and transmission uniformity.
The Chroma 61008 CFP/YFP/dsRed 630/60 Filter is a reliable and versatile tool for researchers working with fluorescent proteins in a variety of cellular and molecular studies. Its optimized excitation and emission spectra make it an ideal choice for multicolor fluorescence microscopy, enabling researchers to gather valuable data with minimal background noise and cross-talk.
1. High-Quality Filters: Chroma is known for its high-quality filters, ensuring minimal fluorescence bleedthrough and improving the quality of your images.
2. Broadband Filters: The 630/60nm bandpass filters are broadband, which means they can accommodate a range of emission wavelengths for each fluorescent protein (CFP, YFP, DsRed). This versatility can be beneficial in a lab setting.
3. Reduced Photobleaching: The filters are designed to minimize photobleaching, which can extend the lifespan of your fluorescent proteins and increase the efficiency of your experiments.
Cons:1. Cost: High-quality filters like the Chroma 61008 can be quite expensive. This might be a concern for researchers or labs with limited budgets.
2. Potential Waste: Since the filters are specific to each fluorescent protein, if you're working with a variety of proteins, you might end up with filters that are only used occasionally, leading to potential waste.
3. Limited Compatibility: These filters are designed for specific excitation sources and may not be compatible with all microscopes or imaging systems.
Conclusion: The Chroma 61008 CFP/YFP/DsRed 630/60 filter offers high-quality, broadband filters that can help improve the quality of your images and reduce photobleaching. However, its high cost and potential waste could be drawbacks for some users. If you're working with a specific set of fluorescent proteins and have the budget, this filter could be a valuable investment. If you're on a tight budget or work with a variety of proteins, you might want to consider more cost-effective alternatives or filters that offer greater compatibility.
From a retired Columbia U lab Discontinued.